2D-PCR: a method of mapping DNA in tissue sections

2D-PCR: a method of mapping DNA in tissue sections.

A novel approach was developed for mapping the location of target DNA in tissue sections. The method combines a high-density, multi-well plate with an innovative single-tube procedure to directly extract, amplify, and detect the DNA in parallel while maintaining the two-dimensional (2D) architecture of the tissue. A 2D map of the gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was created...

Benjamin Shapiro 2 D - PCR : A Method of Mapping DNA in Tissue Sections 37 2 Quan Zhong A High - Throughput Platform

Quantifying mRNA levels across tissue sections with 2D-RT-qPCR.

Measurement of mRNA levels across tissue samples facilitates an understanding of how genes function and what their roles are in disease. Quantifying low-abundance mRNA requires a workflow that preserves spatial information, isolates RNA, and performs reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR). This is complex because these steps are typically performed in three separ...

DNA Measurement of Overlapping Cell Nuclei in Thick Tissue Sections

The paper describes an improved image analysis procedure for measuring the DNA content of cell nuclei in thick sections of liver tissue by absorption densitometry. Whereas previous methods only permitted the analysis of isolated nuclei, the new technique enables both isolated and overlapping nuclei to be measured. A 3D segmentation procedure determines whether each object is an isolated nucleus...

Detection of Bovine Leukaemia Virus Proviral Dna in Paraffin Sections, Tissue Smears, and Peripheral Blood Leukocytes by in Situ Pcr

In situ PCR was developed for the detection of proviral DNA of bovine leukaemia virus in paraffin embedded tissues, tissue smears of experimentally inoculated sheep, as well in peripheral blood leukocytes of naturally infected cattle. The liver, spleen, lung, heart, kidney, and lymph node were collected from two infected sheep, which died or were euthanized due to lymphosarcoma and persistent l...